How do you isolate hydrocarbon degrading bacteria?
The hydrocarbon degraders which are stored on glycerol stock were subjected to its efficiency of crude oil degradation. The isolates were single streaked on BHA plates overlaid with 100 µl of crude oil and on plain NA plates and were incubated at 25°C for 14 days and 37°C for 24 – 48 hrs respectively.
What is hydrocarbon degrading bacteria?
Petroleum hydrocarbon-degrading bacteria are ubiquitous in nature and can utilize these compounds as sources of carbon and energy. Bacteria displaying such capabilities are often exploited for the bioremediation of petroleum oil-contaminated environments.
What is hydrocarbon bioremediation?
Bioremediation relies on microbial metabolic activities in the presence of optimal ecological factors and necessary nutrients to transform organic pollutants such as petrogenic hydrocarbons.
What is the significance of isolating hydrocarbon degrading microorganisms?
Thus, the isolation and selection of hydrocarbon-degrading microorganisms are important steps in a bioremediation program. From microbial isolates, it is possible to study in more detail the enzymes, metabolic pathways, and environmental factors involved in hydrocarbon biodegradation.
What are hydrocarbon utilizing bacteria?
Five hydrocarbon utilizing bacterial species were identified as Staphylococcus saprophyticus, Bacillus amyloliquefaciens, Pseudomonas aeruginosa, Comamonas testosteroni and Chryseobacterium cucumeris while five hydrocarbon utilizing fungal species were identified as Penicillium citrinum, Penicillium brocae, Fusarium …
Can we isolate hydrocarbon degrading bacteria from oil polluted soil?
The purpose of our study was to isolate, screen and identified the hydrocarbon degrading bacteria from oil polluted soil. Three oil contaminated soil were collected from Arzew oil refinery, Northwest of Algeria.
What is the role of microbes in the degradation of hydrocarbons?
The use of microbial metabolism in the degradation of some aliphatic and aromatic compounds plays a key role in ridding the environment of hydrocarbon residues.
Are functional genes in hydrocarbon-utilizing bacteria from the rhizosphere catabolic versatile?
This work assessed the catabolic versatility of functional genes in hydrocarbon-utilizing bacteria obtained from the rhizosphere of plants harvested in aged polluted soil sites in Ogoni and their attenuation efficacy in a bioremediation study. Rhizosphere soil was enumerated for its hydrocarbon-utilizing bacteria.
Do hydrocarbon degraders stored on glycerol stock undergo crude oil degradation?
The hydrocarbon degraders which are stored on glycerol stock were subjected to its efficiency of crude oil degradation. The isolates were single streaked on BHA plates overlaid with 100 µl of crude oil and on plain NA plates and were incubated at 25°C for 14 days and 37°C for 24 – 48 hrs respectively.