Which protein is used for termination of replication?
The replication termination protein or replication terminator protein (RTP) is one of only two well-defined proteins known to be involved in arresting DNA replication forks, the other being a protein known as Tus (termination utilisation substance or DNA replication terminus site-binding protein or ter-binding protein) …
Is Tus a DNA-binding protein?
Tus, a DNA-binding protein, mediates arrest of DNA replication in Escherichia coli. Tus binds to DNA sequences called Ter sites, located in the terminus region of the chromosome, and forms replication-arrest complexes that block movement of DNA replication forks in a polar fashion.
What role does the Ter Tus complex play in E coli DNA replication?
The high-affinity binding of the Tus protein to specific 21-bp sequences, called Ter, causes site-specific, and polar, DNA replication fork arrest in E coli. The Tus-Ter complex serves to coordinate DNA replication with chromosome segregation in this organism.
What does the Tus protein do?
Tus, also known as terminus utilization substance, is a protein that binds to terminator sequences and acts as a counter-helicase when it comes in contact with an advancing helicase. The bound Tus protein effectively halts DNA polymerase movement. Tus helps end DNA replication in prokaryotes.
What is the function of Tus protein quizlet?
What is the function of Tus protein? It allows the replication forks to be stopped by the ter sites in both orientations. Although the replication fork moves 10x faster than RNA polymerase, the Tus protein acts to make it move even faster. It stops transcription when the replication machinery is approaching.
What terminates the process of DNA replication?
DNA replication ends when converging replication forks meet. During this process, which is known as replication termination, DNA synthesis is completed, the replication machinery is disassembled and daughter molecules are resolved.
What protein is required for connecting Okazaki fragments?
Which of the following protein is required for connecting Okazaki fragments? Explanation: After initiation, the chain elongation and joining of Okazaki fragments take place by DNA gyrase, DNA ligase, DNA polymerase. 8.
Which sequence is required for binding of Tus protein?
The Tus protein regulates tus gene expression by binding to the TerB sequence and blocking the initiation of transcription of tus.
What is the function of the Tus protein?
What is the function of Tus protein group of answer choices?
What is DNA termination?
Termination of DNA replication occurs when two replication forks meet on the same stretch of DNA, during which the following events occur, although not necessarily in this order: forks converge until all intervening DNA is unwound; any remaining gaps are filled and ligated; catenanes are removed; and replication …
What is termination in DNA replication in eukaryotes?
In eukaryotic cells, termination of replication usually occurs through the collision of the two replicative forks between two active replication origins. The location of the collision varies on the timing of origin firing.
Which enzyme is used for joining of Okazaki fragments?
DNA ligase
The last deoxyribonucleotide is joined by a different enzyme, DNA ligase, which uses one ATP to join the Okazaki fragment into the growing lagging strand.
How Tus protein perform the termination of DNA replication?
How TUS protein perform the termination of DNA replication?
What is termination in DNA replication?
Which protein is required for connecting Okazaki fragments?
Which protein joins together the Okazaki fragments?
Which protein joins together the Okazaki fragments of DNA in the lagging strand? DNA ligase joins the Okazaki fragments in the lagging strand, turning it into a continuous strand.