What is pl4440?
coli strains for the induction of RNAi by feeding in C. elegans . Inserts that will correspond to the dsRNA that will trigger RNAi are cloned into the polylinker shown. The T7 promoters are symmetric; thus, the orientation of the insert is unimportant.
What type of molecule triggers RNA interference?
Endogenous triggers of RNAi pathway include foreign DNA or double-stranded RNA (dsRNA) of viral origin, aberrant transcripts from repetitive sequences in the genome such as transposons, and pre-microRNA (miRNA).
Why is RNAi used?
RNAi may be used for large-scale screens that systematically shut down each gene (and the subsequent proteins it codes for) in the cell, which can help to identify the components necessary for a particular cellular process or an event such as cell division.
What is RNAi triggered by?
How many Addgene sequences are in the l4440 series?
L4440 Sequences (4) Addgene Sequences: Based on next-generation sequencing (NGS) results where indicated (Addgene NGS Result), or assembled from reference sequences and/or Sanger results (Addgene Assembled Sequence).
How is plasmid DNA eluted in a QIAGEN test kit?
RNA, proteins, metabolites, and other low-molecular-weight impurities are removed by a medium-salt wash, and pure plasmid DNA is eluted in high-salt buffer (see flowchart ” QIAGEN Plasmid Kit procedures”). The DNA is concentrated and desalted by isopropanol precipitation and collected by centrifugation. See figures QIAGEN Plasmid Kit procedures.
How is plasmid DNA eluted from the tip?
The plasmid DNA is first treated with endotoxin removal buffer ER, and then applied to QIAGEN’s anion-exchange tip. After performing a wash step, the plasmid DNA is eluted from the tip, followed by isopropanol precipitation and redissolving the DNA in a suitable volume of endotoxin-free buffer TE. FAQ ID -500
How can I isolate plasmids from Streptomyces spp?
Isolation of very low-copy plasmids from Streptomyces spp. using the QIAGEN® Plasmid Maxi Kit – (EN) EN PDF(123KB) The procedure has been used successfully for isolation of plasmids SCP2 and SCP2* as well as the plasmids listed in Table 1 (see next page) from S. coelciolorand S. lividansstrains.