What is LB agar plate?
About LB agar plates Luria-Bertani (LB) agar plates are nutrient-rich plates used for growing bacteria on. They are commonly used during cloning experiments to amplify antibiotic-resistant, competent bacteria. The same components, excluding agar, can be used to create a liquid LB media.
How do you make 1000x chloramphenicol?
Directions:
- Dissolve 0.34 g of chloramphenicol into 10 ml 100% ethanol.
- Filter through a 0.22 µm filter to sterilize.
- Aliquot and store at -20°C.
- Use at 1:1000 dilution in LB or LB-Agar.
What concentration is 1000x?
1000x concentration just means your stock is 1000x more concentrated than your working concentration. For example, if your stock is 50 mg/mL the working concentration is 50 ug/mL. To go from one to the other you dilute your stock 1000x, so for 1.5 mL you would add 1.5 uL.
How do you make a 1000x solution?
Preparation of 1000X Antibiotic Stock Solution
- Weigh x grams of antibiotic-of-interest.
- Dilute in pure 100% ethanol for chloramphenicol or sterile deionized water for kanamycin and ampicillin.
- Syringe filter water-based antibiotic solution using a 0.22-μm filter.
What is agar LB?
LB agar is LB broth that contains agar and is typically prepared in petri dishes. The flat, solid properties of LB agar allow the plating of bacterial cultures and generation of colonies.
How many agar are in a pound plate?
Dissolve 15 g of Bacto agar in 1.0 L of LB medium and sterilize by autoclaving. Cool to 50°C in a temperature-controlled water bath.
How many lbs is 8 plates?
4 Plates 225 lbs. 6 Plates 315 lbs. 8 Plates 405 lbs.
What is the final concentration of chloramphenicol in lb?
Chloramphenicol – The frozen stock solutions of chloramphenicol are at 25mg/ml in 100% ethanol and are marked with purple. The final concentration for LB liquid culture is 25mg/ml. To obtain this in 100ml of LB, add 100ul stock solution.
What is the final concentration of tetracycline and chloramphenicol?
Tetracycline – add 1ml tetracycline stock (at 15mg/ml) per liter of agar to obtain a final concentration of 15ug/ml. Mark the plates with a single black line on the side. Chloramphenicol – add 1ml chloramphenicol stock (at 25mg/ml) per liter of agar to obtain a final concentration of 100ug/ml. Mark the plates with a single purple line on the side.
How do you prepare chloramphenicol from frozen stock solutions?
Chloramphenicol – The frozen stock solutions of chloramphenicol are at 25mg/ml in 100% ethanol and are marked with purple. The final concentration for LB liquid culture is 25mg/ml. To obtain this in 100ml of LB, add 100ul stock solution. Follow the recipe card in box for making LB plates, being sure to add the agar.
What is the final concentration for lb liquid culture?
The final concentration for LB liquid culture is 50 ug/ml. To obtain this in 100ml (the amount in each LB bottle), add 100ul of 50 mg/ml stock solution OR 50ul of 100 mg/ml stock solution.