What does SYBR green contain?
SYBR® Green master mixes are 2x concentrated, and contain dNTPs, MgCl2, and DNA polymerase. SYBR® Green master mixes are available with other dyes including ROX. Many PCR machines require a passive reference dye.
What does SYBR green do?
SYBR Green I is the most commonly used fluorescent dye. It binds specifically to double-stranded DNA. Using this dye, double-stranded DNA molecules can be exclusively quantified in the presence of single-stranded DNA molecules during denaturation experiments.
Is SYBR green hot start?
USB HotStart-IT SYBR Green qPCR Master Mix uses a novel hot start method designed and developed at Affymetrix called primer sequestration. With this method, a protein binds and sequesters primers at lower temperatures making them unavail- able for use by Taq DNA Polymerase.
How does SYBR Green assay work?
SYBR® Green chemistry is a method for performing real-time PCR analysis. SYBR Green dye binds the minor groove of double-stranded DNA. When SYBR Green dye binds to double-stranded DNA, the intensity of the fluorescence increases. As more double-stranded amplicons are produced, SYBR Green dye fluorescence increases.
How does SYBR Green qPCR work?
SYBR Green is one of the most commonly used fluorescent dyes in qPCR. It binds to double-stranded DNA molecules by intercalating between the DNA bases. Once intercalated to DNA, SYBR Green becomes less mobile, causing its energy to be released as fluorescence.
How does SYBR Safe stain DNA?
SYBR Safe is a cyanine dye used as a nucleic acid stain in molecular biology. SYBR Safe is one of a number of SYBR dyes made by the Life Technologies Corporation. SYBR Safe binds to DNA. The resulting DNA-dye-complex absorbs blue light (λmax = 509 nm) and emits green light (λmax = 524 nm).
Why is SYBR Safe Safe?
SYBR®Safe is a commercial DNA stain manufactured by Invitrogen. It is marketed as being less harmful than ethidium bromide, but this is debatable. Its major advantage is that it is as sensitive as ethidium bromide but does not require UV light for visualization.
How does SYBR Safe bind to DNA?
However, since DNA is clear and colorless, the bands cannot be seen with the naked eye. SYBR Safe® is a fluorescent DNA stain that binds specifically to the DNA double helix. When excited with UV or blue light, any SYBR Safe® that is bound to DNA fluoresces with a bright green color.
How do you use SYBR Safe DNA stain?
- 1.1 Soak the gel in SYBR Safe™ stain. If using SYBR Safe™ gel stain concentrate, dilute 10,000X in TAE or TBE buffer (as appropriate) prior to use.
- 1.2 Incubate for 30 minutes.
- 2.1 Prepare the agarose gel directly in SYBR Safe™ DNA gel stain.
- 2.2 Run the gel.
Why Applied Biosystems® SYBR Green PCR master mix?
Applied Biosystems® SYBR® Green PCR Master Mix combines SYBR® Green I dye, AmpliTaq Gold® DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial. Applied Biosystems® SYBR® Green PCR Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan® probes are required.
What are the different Biosystems brands?
Our innovative life science, applied science, and clinical products are now found under the Applied Biosystems™, Invitrogen™, Gibco™, Ion Torrent™, and Molecular Probes™ brands.
What happened to Life Technologies?
Life Technologies Life Technologies was acquired by Thermo Fisher Scientific in January, 2014 Our innovative life science, applied science, and clinical products are now found under the Applied Biosystems™, Invitrogen™, Gibco™, Ion Torrent™, and Molecular Probes™ brands. Home page Shop all products
What are the components of the SYBR Green 1 product?
It contains the following components: SYBR Green 1 dye, AmpliTaq Gold DNA polymerase, dNTPs (with dUTP), Passive Reference 1 and optimized buffer components; all the necessary components to perform real-time PCR in a convenient premixed 2X solution. This product has been tested on and is supported for use on an ABI PRISM Sequence Detection System.