What is standard plate counting?
The Standard Plate Count (SPC) means the colony count of the mesophilic bacteria growing under aerobic condition on standard methods agar (Plate Count Agar), and SPC becomes the representative index indicating the degree of the microbial contamination of the food.
What is the principle of standard plate count method?
The standard plate count method consists of diluting a sample with sterile saline or phosphate buffer diluent until the bacteria are dilute enough to count accurately. That is, the final plates in the series should have between 30 and 300 colonies.
Is standard plate count same as total plate count?
The aerobic plate count (APC) is also known as standard plate count, aerobic mesophilic count, total plate count or aerobic colony count. The APC is used to estimate the bacterial population in a food sample.
Why is CFU used in plate counts?
Not all bacterial cells produce colonies, as some bacteria tend to clump or aggregate, and some are nonviable. For this reason results are reported as colony forming units (CFU)/ml of bacterial culture. Ideally only plates with 25-250 colonies are used.
What is standard plate count in milk?
The Standard Plate Count (SPC) of a producer raw milk samples gives an indication of the total number of aerobic bacteria present in the milk at the time of pickup. Milk samples are plated in a semi-solid nutrient media and then incubated for 48 hours at 32°C (90°F) to encourage bacterial growth.
Why do we multiply direct counts by 50000?
The factor of 50,000 is used in order to determine the cell count for 1 ml: 1 ml = 1000 mm3 = (50 times the chamber depth of 0.02 mm) * 1000. You Might Like These Articles: Branches of Microbiology.
What is an acceptable CFU ml?
This level of sensitivity is unacceptable when the maximum allowable limit for microorganisms is 200 CFU/mL.
What are some of the disadvantages of the standard plate count?
oped because of certain disadvantages of the standard plate method. Chief among these disadvantages are: (1) the length of time required for incubation, 48 hours being the shortest; (2) large amount of glassware and culture medium required, as well as other expensive equipment, and (3) the paucity of details regarding the kinds of organisms present.
What is the purpose of the standard plate count?
Saline of buffer
Is standard count and total plate count is same?
The standard plate count, sometimes also referred to as the total plate count, is probably the most widely used technique for evaluating microorganisms in foods. The purpose, as its name implies, is to estimate the number of viable microorganism cells in a given sample of food. Likewise, what is the standard plate count method used for quizlet?
What is the difference between plate count and total count?
Total Plate Count (TPC) This is a count of microbial load in a sample i.e. it an enumeration of all heterotrophic bacteria that will grow in aerobic or microaerophilic conditions that will grow at 35 0 C. This plate count will tell you how good your sanitization plan is, measure the safety of your product or water supply.